How to Copy and Paste

Below is a free essay on â€Å"Copy And Paste† from Anti Essays, your source for free research papers, essays, and term paper examples. Behind every great man is a beautiful, charming maiden who holds his heart. What if this woman was not absorbed with taking care of his heart but was completely absorbed with money, reputation, and her own needs. In Fitzgerald's The Great Gatsby, Mrs. Daisy Fay Buchanan is the object of affection or the â€Å"rock of [Gatsby's] world. â€Å"(99) All Daisy's life she has wanted to be noticed, to be heard, and to be loved. However, when everything she has always anted is being held in her hands, in the form of Gatsby, Daisy chooses money as her form of happiness ultimately leading to her misery. Daisy's action and choices are extremely defined by her â€Å"East egg† way of life, which is a representation of old money and high class society within the novel. In short, Daisy thinks with her wallet instead of using her common sense, her he ad, or her heart. When reading the novel â€Å"it [seems]†¦the thing for Daisy to do [is] to [leave Tom], child in arms†(20) but unfortunately â€Å"there are no such intentions in her head†.The reason being, Tom is her financial prov†¦ †¦ middle of paper †¦ †¦ aisy is the definition of charm and beauty, she will never allow herself to hold his heart. Daisy's love for money, her reputation, and her own needs have ultimately led to her down fall. Daisy chose to marry Tom and his wealth over being Gatsby's foundation of love. Daisy believed money would give her the attention, giver her the voice, and give her the love she wanted all her life. However, all she has received from pursuing money is misery. Works Cited Fitzgerald, F. Scott. The Great Gatsby

Lord of the Flies Paper Essay

What is stronger savagery or civilization? The human has two desires that conflict with each other: to live by civilization and to live by savagery. The civilized impulse we have is to live peacefully, morally, and by rules and laws. The savage characteristic we have is to act violently, using force to gain authority and power over others. In Lord of the Flies by William Golding, symbolism and figurative language links the clash of civilization and savagery with a group of boy are stranded on an island. William Golding inserts symbolism into his story Lord of the Flies to elaborate on savagery vs. civilization. For example, at the beginning of the story civilization is still intact, â€Å"That’s what the shell’s called. I’ll give the conch to the next person to speak. He can hold it when he is speaking,† (33). Ralph and Piggy discover the conch shell on the beach and decide to use it for organization and order. The conch soon becomes a symbol of civilizati on as the shell effectively governs the boy’s meetings. The boys create a society on the island, â€Å"The twins, Sam ‘n Eric were the first to get a likely log but they could do nothing till Ralph, Jack, Roger and Maurice found room for a hand-hold,† (39). The relationship develops between the older and younger boys when the older ones and leaders and the younger ones are followers. The younger boys emphasize the older boy’s connection with civilization savagery. In Lord of the Flies by William Golding also introduces figurative language when describing the conflict of savagery and civilization. A type of figurative language, such as foreshadowing, is utilized, â€Å"It’s time some people knew they’ve got to keep quiet and leave the deciding things to the rest of us,† (102).The foreshadowing of the possible dictatorship presents itself in this quote. Jack’s violent actions and words dictate the boys and without conch savagery has come about. For instance Golding creates a metaphor, â€Å"The sun was right and the danger had faded with the darkness,† (108). The reassurance and relaxation come back with the dark and the night. The metaphor establishes that when the darkness approaches the danger disappears because essentially the savages are sleeping. The central concern of Lord of the Flies is the conflict between two competing urges that exist within all human beings: to act peacefully or violently. In the end, civilization can quickly turn to savagery. People’s actions, words and beliefs can turn any situation into a bad one or a savage one. Savagery vs. civilization is in everyday life and it is the way we handle things that decides how society works.

Favourite Hoiliday Essay

The word Diwali is an incorrect form of the word Dipawali, which means Rows Of Light. People will celebrate this festival during Karthik (October- November). All Indians will celebrate this festival but different religious people have various opinions about it. The Hindhus celebrates this festival as a gesture of their joy because when Sri Ramachandra return to Ayodhya after 14 years exile in the forest following his victory over evil demon Ravana in a war and signifies the victory of good over evil. This is a day for which every child waits through out the year. Though it’s a festival for all people it is very interesting to young people because they savor most on this day. On this occasion everyone will like to wear traditional dresses. On this day my house is completely decorated with flowers ,candle lights and elecronic bulbs so it looks like a heaven twinkling with sparkling lights. I initiate shopping around 1 month before the festival, because it is not only a festival for us it’s a day where all of our cousins compete to look pretty, so it resembles the fashion show. Everyone will peer like angels dropped from heaven, I think two eyes are not enough see the beauty of them. On this ceremony my mother will prepare delicious food, the menu itself is really mouth watering. My favourite dessert is double ka meeta it’s very rich and delicious. The ingrediants added in this are bread, milk, sugar, butter, almonds and cashews. One of the only thing I miss about Hyderabad apart from people is food especially this dessert nothing beats this taste. when I eat this dessert it definitely comes into my dream because its taste is that superb. The thing that which sounds very interesting and elite part of this festival is bursting of crackers. There are plenty kind of crackers, but I like rocket and Vishnu-bar most. I am really excited while bursting rocket because it directly goes upwards with vivid colors and zooyi sound. It’s really a beautiful scene when all the crackers bursted and emits bright colours with variety sounds. In the night time the whole city looks like a stars in the sky. All in all, I go to bed with all these memorable moments in my mind. It brings a wonderful feeling into the heart and souls of every man, woman and  child. Everyone must celebrate the festival and savor the fresh feeling in it. New clothes, dishes, sweets, fun and crackers all these are part of this festival.

Engagement Exercise Create and Explain your own Example Essay

Engagement Exercise Create and Explain your own Example - Essay Example In my capacity as an Asian, I would like to point out that I was introduced to a very good culture. All along, I have learnt to respect my culture and use it to define my religion, language, communications and relationship with other people. I always know that I should relate well with other people since my closeness with them can help me a great deal. Also important, is the respect, acceptance, understanding and appreciation of other people’s culture. Since my childhood, my parents have been telling me that I should be ready to respect other people even if they have different cultural beliefs from my own. One day, when I was in college, I met a man from a different culture. So, despite coming from a conservative community that restricts the relationship between people of different genders, I had to greet him without any fear. Also, I had to take my time to be close to him and discuss a lot with him. Even if I knew that this is something not accepted by my culture, I had to do it because of my colleague who comes from a different culture. It was a good thing to do because it showed respect and enabled us to communicate so well without any barrier. Since that day, I have been effectively communicating with people from different cultural backgrounds. My experiences demonstrate how the acceptance, understanding and tolerance of other people’s culture can be of great contribution to the communication process. This has made me to be an effective communicator who relate so well with other people. As a communication expert, I know that intercultural understanding is necessary for a healthy relationship between different people. Due to my free interactions, I have managed to learn different aspects of other people’s cultural traditions. This has enabled me to know how to use verbal and non-verbal communication strategies. At the same time, it has prepared me to be a critical listener who does not only lend my

The right of abode in Hong Kong Essay Example | Topics and Well Written Essays - 1250 words

The right of abode in Hong Kong - Essay Example The government ought to review the population policies, with picky references to mainland mothers birthing in Hong Kong city. Many lawmakers would say that these babies born here not only strain obstetric services for local parents, but also put pressures on healthcare, education, welfare, and housing services in the city. The government should consider responding that the question of children born of parents of both non-Hong Kong lasting residents can be viewed positively as they may turn into new blood of the aging population. The government should review the population policies suiting for long-term growth. The government should question if children mothered by nonlocal parents could actually relieve the aging population question. Again the Hong Kong SAR government should strive for the authorities to vet the daily ration of 150 individual-entry permit hopefuls. The government should take a look back to the chief secretary-led direction-finding committee for not making an announcement of any population policy reviews, leaving this population policy to fail to keep up to the current state. Interpretation of the Basic Law The government needs to note that the local population could increase by about 90,000 every year (with 40,000 of these children born of nonlocal parents). It should be worried the situation might be out of control and therefore need to seek for an interpretation of the applicable Basic Law provisions on residence rights of such category of children. Conversely, the Special Administrative Region (SAR Government) lacks continuing plans in its allocation and governance of finances, resulting in makeshift policies (Siu and Ku, 2008). Citizens believe the final way

Characteristics of Early Christianity Movements Essay

Characteristics of Early Christianity Movements - Essay Example He and his disciples lived an eremitical life, one of the primary types of monasticism. As part of living the eretical life, St Anthony and his followers vowed living in austerity far away from the world. They commit themselves in answering the needs of the soul over the body by prioritizing activities that nourish the soul instead of the physical needs. (Bacchus) According to Athanasius, Bishop of Alexandria, St Anthony, who adhered to eremitical monasticism, fearlessly battled myriad temptations of the devil. In this regard, his disciples were enjoined to center their prayers on seeking the Lord's guidance through constant prayers to win over the evil one and learning the discipline of discerning spirits to recognize deceitful ways to sin. (Schaff & Wace) Another type of monasticism that characterized the early Christian movement is the cenobitical life. This type of monasticism was revived by St Benedict, who composed a new set of rules. From these rules, it could be observed that St Benedict's viewpoint does not involve extraordinary asceticism and narrow mindedness. As such, these sober regulations were based on common sense with emphasis on collectivism instead of individualism. Cenobitic monasticism also emphasized family spirit and discouraged all private ventures.

Product and Pricing Strategy Essay Example | Topics and Well Written Essays - 1250 words

Product and Pricing Strategy - Essay Example te a variety of industrial and office-based computer and laptop solutions, along with state-of-the-art services, software collaborations and innovative accessories. EJ’s Innovations and More has a wide customer base for all of its electronic product offerings. This product will make full use of access to the existing customer base as they are all potential users of computers and laptops. Furthermore, they are already familiar with the brand name of EJ’s and have the trust relationship and faith in the product quality and service assurance of the company. With this product, the company is planning to target all computer and laptop users. The potential customers range from 14 year old girls and boys to aged, retired gentlemen and ladies. However, this plan outlines the target audience, described later, for this particular wave of marketing. The strategy of EJ’s Innovations and More is to offer customers a wide range of personal computers and laptops. These will be available in a variety of designs and colors, suiting the different needs and whims of the customer. They will be available in different varieties, starting with the basic computer or laptop, to the more advanced versions with greater memory capacity, RAM, accessories and additional features. The most important aspect of these computers will be that with registration, they will be ready for immediate use. They will work easily on all software platforms from other companies with minimal installation. To assist the customer, customer support teams will be available 6 days a week and helpline will be open 24/7 to answer any product related questions. The computers and laptops offered by the company will all carry the same brand promise of EJ’s which is one of complete innovation and convenience, designed to make the life of the customer easier and happier. The specific target audience of this plan includes the people aged between 40 to 70 years, especially those with some sort of impairments or

Qualitative & Quantitative Designs Essay Example | Topics and Well Written Essays - 500 words

Qualitative & Quantitative Designs - Essay Example Both research designs are suitable for establishing how lack of communication is increasingly becoming a business problem within non-profit organizations. However, each approach has inherent strengths and weaknesses that make them suitable or not to solve the business problem. Establishing the issue of miscommunication in non-profit organizations can be successful due to the strengths associated with the qualitative research design. Such a design gives insight and explores the significance of miscommunication as a business problem. For example, the article uses the grounded theory approach, which is a qualitative research design. It is evident that qualitative research design is holistic as it approaches the issue right from its stem to its effect on business performance. It involves interviewing and reviewing the records held by the non-profit organization. The results are supported by the data obtained from interviews and observations. However, the method is quite broad. It covers a wide area and even includes items that have not been affected by the business problem. It takes a lot of time to research the issue at hand because the researcher had to formulate, test, and establish propositions until the problem is established. On the other hand, the quantitative research design successfully establishes the problem and dissects is cause and effect relationship. In addition, the method allows relationships to be tested, hence it is objective. The method is time saving or reductionistic as it only test items that are affected. The aspects of business performance affected by miscommunication include high staff turnover and reduced corporate social responsibility functions. The method tests theories and employs statistical analysis of figures. However, the method is limited by the fact that it generalizes items in an attempt to establish the cause and effect relationship. It might not be

Supreme Court Essay Example | Topics and Well Written Essays - 500 words - 1

Supreme Court - Essay Example The problem, however, appears to lie in whether the children are given the appropriate moral guidance by their parental figures. Perhaps Vermont is too complacent in their viewpoints that children will naturally evolve into moral adults, thus there is no emphasis given in this aspect of guiding juvenile behaviors. This may have to do with the social class of the Vermont citizens, who are mostly middle- to upper-class who may just instinctively believe that these violent crimes simply could not be committed by their well-to-do sons and daughters. Powers describes the ease of acquiring heroin in the state, which may also contribute to the violent crime ratios and is associated with social class status, as these well-to-do youths can easily afford the drug prices. The team appears to have mixed thoughts about whether the juvenile should be given the death sentence, highlighting the strong division in the country about what to do with juvenile violent crime offenders. It appears that Powers believes that many of the failures of todays children in these affluent neighborhoods stems from parental complacency and an inability to address that violence and teenage angst occurs routinely regardless of their state of origin. Powers, it appears, believes that most of the fault lies with those who are in charge for their failures in molding positive childhood behaviors. In reference, specifically, to the New Hampshire murders, it seems that Powers is linking their murderous intentions with failure of parents to recognize the difficulties (and respond to them correctly) facing children of these teenage years. It does not appear that Powers considers the death penalty as the proper course of action, rather counseling and parental involvement is the best method. Powers points out that small towns, like any other city, maintains their fair share of juvenile-related crime, suggesting

Religion is the Cause of War Essay Example | Topics and Well Written Essays - 1000 words

Religion is the Cause of War - Essay Example They may have heard the news about religious wars and conflicts, and they themselves may have come to believe that religion is the reason behind civil war conflicts and even internationally known incidents like the 911 terror attacks on the World Trade Center in 2001. Nevertheless, they may not exactly know why this is so or what exactly is going on in the minds of religious terrorists or those people who wage war and conflict in the name of religion or God. The paper is therefore intended for them in order to enlighten them and strengthen their belief in the idea that religion is indeed a significant and primary cause of war. The reasons in order to establish this thesis are to be presented as ideas of individual experts on the topic. The expected response is a greater awareness of the idea and development of a mind which is independent of religious influence. The less religion can get into one’s psyche, the less chances it will encourage one towards conflict. The point that the presentation is aiming at is that sociologically and psychologically, religion is a major cause of war. According to Jonathan Fox (2004), who is a lecturer of political studies in Bar-Ilan University in Maryland, and who was Ph.D. in Government and Politics, which he received from the University of Maryland, religion causes war because it is modernity that has triggered its role. Although many theorists thought that religion would disappear or would somehow dissolve into something insignificant because of the advent of modernization, religion has somehow even attained a stronger role in society more than ever. There are various reasons and evidence presented by Fox in order to establish his point. First, efforts at modernization have actually failed in many countries of the Third World where modernization was introduced. The western secular ideologies introduced by modernization have actually caused a backlash in these areas of the world. The reason for this is the strong religi ous culture of the Third World, which is mostly tribal in nature and mostly highly specific among natives, which means that they may not include an extremely large community of believers like Christians. Actually, tribal groups like those of African tribes and even some cults have members who are more loyal to their groups than those belonging to large religions like Christianity and Islam. This is something that proponents of modernization failed to see. Modernization has actually therefore failed to anticipate the role of traditional lifestyles, morals and community values. These things actually strengthen the community and religious spirit of the people who are members of these religious tribal groups. That is why the more these groups are threatened by modernization, the more religious they will tend to be and the more violent or defensive they may become in order to defend their identity. This means that anything that condemns or threatens the stability of these small religions is actually conflict. The more these religions are threatened, the more violent the members become and the more they tend to cause conflict and war. One last point that Fox (2004) emphasizes is that the newfound freedom that democracy has afforded the common individual when it comes to choosing his religion has actually made people increase in their religiosity. This means that people who have freely chosen their systems of faith actually have a greater reason for defending their religion for defending it means defending their choice, as choice often reflects one’

School and Society- assignment Essay Example | Topics and Well Written Essays - 1500 words

School and Society- assignment - Essay Example e and size of the American society and its level of diversity, education has a broad role to play in addressing vital issues such as the levels of education in the ethnic and underprivileged sections of the society. It is next to impossible to do so without understanding the ramifications of the range of social and administrative measures in the aid of education, adopted by the American society from time to time. Q 1. Describe the perspectives of liberal and vocational education in the development of the comprehensive high schools. Provide examples of how each has helped to shape the role of education in the United States. How do they continue to impact contemporary education? Ans. Considering the rapid changes being unleashed in the 21st century and the basic societal issues, challenges and opportunities that the students have to contend with in an American and a global context, it is important to seriously meditate on the ingredients of the curriculum that will enable the students to lead a positive, productive and meaningful life. Thus it is imperative to expose the students to a wide range of disciplines (U.S. Newswire 1). In that sense, liberal education has played a groundbreaking role in the development of comprehensive high schools in the US. The augmenting need for increasing pragmatism in the 21st century America cannot dilute the importance of traditional liberal education in the schools. However, one cannot underestimate the need for vocational education in schools. Vocational education in tandem with liberal education prepares the individuals to be productive leaders and workers who adhere to a sound ethical background. The signing of the Voc ational Education Act by President Lyndon Johnson gave way to the most comprehensive vocational education program in the history of America. Q2. Compare the major educational recommendations of James B. Conant with those advocated by Mark Van Doren in the article â€Å"Education for All†, assessing their relative

Research Essay Example for Free

Research Essay El Fili as it is popularly called, is a darker and more evolved novel compared to Noli. Its a book about revenge, manipulation, deception and greed to name a few. Its a shorter book. The dreaminess and idealism of the Noli Me Tangere gives way to the darker, grimmer, more cynical tone of the El Filibusterismo. So the language style also changes instead of the longer, declamative dialogue of the Noli, you have the more direct, action-packed style of the Fill. The Noli, with its anti-friar commentary, still shows hopefulness in terms of a Just civil colonial governance. In comparison, the Fili describes a deep-seated corruption in oth civil government and church, creating a society seething with injustice, and setting the stage for possible revolution. I guess this definitely shows the changes in Rizals state of mind as he was writing the two novels. So, he wrote Fili and expressed his thoughts on the possible solution to the countrys situation. Yes, if you really analyze the messages in both novels, Nolis intention was to state the problem while Fills was to present the possible solutions. I thought Rizal did not write Fili Just to fan further the flame caused by Noli but to tell the Filipinos he different alternatives to their problems and what he thought was the best solution: non-violence. 1. Style. The opening scene in Noli, the grand welcome party in Capitan Tiagos house mirrors the condition of the country right away. On the other hand, the upper and lower decks in MN Tabo shows the marked difference between the social strata in the country particularly the Spaniards (peninsulares, insulares, mestizos) and the lowly indios. As per as the manner of writing, i. e. , long or short, simple or complex, sentences, rushed or leisurely pacing, they are the same. -rte SCORE: 0-0 2. Tone. Noli is social while Fili is political. Noli still has a heart because it has this passionate love story between Ibarra and Maria Clara. Fili is mostly about revenge and debates which mean it has more brain than heart. So, this depends in the readers preference. I thought that I enjoyedNoli more. Noli over Fili SCORE: 1-0 3. Mood. Noli is confused yet still hopeful. Angst is still underneath. Fillis altogether hatred from page 1 until the last chapter except in the last scene. There are more scenes in Noli where I was mesmerized by Rizals use of beautiful prose putting natural color on his settings. There are still some in Fili but they were overshadowed by the strong hatred emotions from the characters. SCORE: 2-0 4. Diction. Rizal used his characters to express his thoughts. There are these debates or long conversations that while reading, you will get the feeling that Rizal is discussing his views with you. In Noli, there are only 2: Ibarra and Pilisopo Tasyo is one and the debate about the town fiesta as the other. Fili on the other hand, has a lot more: Isagani vs Ginoong Pasta, Macaraig vs Don Cammaro, the friars and government officials in Los Banos, Placido Penitente vs his professor, Isagani vs Padre Fernandez and even the last scene with Simon and Padre Florentino. Wonderful debates. Fili over Noli SCORE: 2-1 5. Point of View. Both employ an omniscient narrator but there is a part in Fili when Rizal used stream-of-consciousness. This is the part when Simoun heard about the death of Maria Clara. What an effective way of expressing Rizals sadness and I could not help but wonder if this was how he felt when Leonor Rivera got married to a foreigner. SCORE: 2-2 6. Narrative Presence. The attitude of the omniscient narrator is more accommodating and patient in Fili rather than in Noli. For example, in the Noche Buena scene in Noli, the narrator used the readers sympathy over the limping Basilio chasing the crazy Sisa and he forgot to describe the setting. In Fili with Basilio after 13 years, I was in awe reading the beautiful description of the forest while Basilio was remembering the first Noche Buena. Narrative Attitude. Despite those beautiful descriptions of the settings in Fill, it is more direct and straightforward. Although the message is sometimes diluted by the many options presented, Rizal was able to tie the loose ends in the very last chapter during the whole-day conversation between Simoun and Padre Florentino. Also, the dialogues are more brutal like in the scene with the talking head or sphinx. SCORE: 2-4 8. Time Frame. Noli starts with Ibarras homecoming after staying in Europe for 7 years. Fili starts witn Simouns appearance atter disappearing as Ibarra atter 1 years. Ibarra is hopeful that he will be able to bring change by putting up a school with his love for his country as the reason. He was able to learn this from his trips abroad including his stay in Spain. However, the 13 years in Cuba was, for me, not enough for Simoun to gather all those treasures that he claimed to have even come rom Marie Antoinette and the pyramids in Egypt. It Just sounded not too plausible.. Time Management. Noli starts at the end of October to December 24 (Christmas Eve). Fili on Christmas Eve to after Holy Week or after the school year. Less than 2 months for Rizal to tell the tale of a small town of San Diego but he needed more than that to tell the events in the city. I thought he used his time frames quite well in both. SCORE: still at 3-4 10. Place. If Calamba were the basis for San Diego, the Binondo and San Diego locations in Noli seems to be more realistic. The Laguna de Bay-Manila-pasig River- Tiyani-Binondo then finally to a provincial place with a plateau and the plateau is right in front of a deep sea is Just a bit of a stretch. How could the weak and wounded Simoun travel to a far-flung provincial place of Padre Florentino? Some say that the basis of this place was Atimonan, Quezon and it is a 4-hour drive from Manila. But for a horse-drawn carriage during the Spanish times? And Simoun was still able to confess to the priest for 1 whole day! SCORE: 4-4 11. Motif. The letters are the recurring images in Noli and there was not a single letter in Fill. Rather, in Fili the recurring images are the treasures and the lights. The letters in Noli denotes secrecy or inner ghosts. They signify the hidden emotional turmoil of Rizal and the Filipinos. The treasures in Fili means evil not all the glitters are gold and the lights, e. g. the stars that were in the sky guiding Basilio, the lights reflected on the surface of Laguna de Bay and the lamplight that could have killed all the rich and famous in Manila, signify hope. Rizal was really wonderful in using his motifs. SCORE: still at 4-4 12. Theme. Overall, Noli is positive: hope, love, beautiful memories, parties, town iesta, picnic by the riverbank, groundbreaki ng for a school, many books, knowledge, etc. On the other hand, again overall, Fili is negative: hate, revenge, death, rape in the open, persecution, students leaving the university, scary stage shows, protests, son killing his own kin, etc SCORE: 5-4 13. Irony. Since there is no prevailing emotion in Noli, the use of irony in that novel is more effective. Who would forget the Christmas Eve when Basilio was chasing Sisa, his lunatic mother? There were also scenes in Filiwhen this was nicely used like when Simoun went to the house of Kabesang Tales and lured him to commit rebellion by howing him the treasures. However, we already knew Simouns intent even at the onset so it was not as effective as those last scene in Noli. The one of Carolino and Tandang Selo was anti-climatic in my opinion. SCORE: 6-4 14. Rythmn. Narrative rhythm is greatly influence by the theme in these two books. I enjoyed the deployment of words better in Noli for example the old-fashioned but kilig dialogues between Ibarra and Maria Clara when they were in the azotea. The many long debates in Fili were at times so convoluted that they tend to go around in circles (not to mention non-sense characters mouthing nonsense statements) that here are parts when they were boring and pointless. SCORE: 7-4 15. Pace. I thought that this was won by Fili by a mile.

Health Essays Alcoholism Drinking Health

Health Essays Alcoholism Drinking Health Alcoholism Drinking Health Definition Alcoholism is an illness marked by drinking alcoholic beverages at a level that interferes with physical health, mental health, and social, family, or occupational responsibilities. Alcoholism is divided into 2 categories: dependence and abuse. People with alcohol dependence, the most severe alcohol disorder, usually experience tolerance and withdrawal. Tolerance is a need for markedly increased amounts of alcohol to achieve intoxication or the desired effect. Withdrawal occurs when alcohol is discontinued or intake is decreased. Alcohol dependents spend a great deal of time drinking alcohol, and obtaining it. Alcohol abusers may have legal problems such as drinking and driving. They may also have problems with binge drinking (drinking 6 or more drinks at one sitting). People who are dependent on or abuse alcohol continue to drink it despite evidence of physical or psychological problems. Those with dependence have more severe problems and a greater compulsion to drink. Alternative Names Alcohol dependence; Alcohol abuse Causes, incidence, and risk factors Alcoholism is a type of drug addiction. There is both physical and psychological dependence with this addiction. Physical dependence reveals itself by withdrawal symptoms when alcohol intake is interrupted, tolerance to the effects of alcohol, and evidence of alcohol-associated illnesses. Alcohol affects the central nervous system as a depressant, resulting in a decrease of activity, anxiety, tension, and inhibitions. Even a few drinks can result in behavioral changes, a slowing in motor performance, and a decrease in the ability to think clearly. Concentration and judgment become impaired. In excessive amounts, intoxication may result. Alcohol also affects other body systems. Irritation of the gastrointestinal tract can occur with erosion of the lining of the esophagus and stomach causing nausea and vomiting, and possibly bleeding. Vitamins are not absorbed properly, which can lead to nutritional deficiencies with the long-term use of alcohol. Liver disease, called alcoholic hepatitis, may also develop and can progress to cirrhosis. The heart muscle may be affected. Sexual dysfunction may also occur, causing problems with erections in men and cessation of menstruation in women. Alcohol affects the nervous system and can result in nerve damage and severe memory loss. Chronic alcohol use also increases the risk of cancer of the larynx, esophagus, liver, and colon. Alcohol consumption during pregnancy can cause severe birth defects. The most serious is fetal alcohol syndrome, which may result in mental retardation and behavior problems. A milder form of the condition which can still cause lifelong impairment is called fetal alcohol affects. The social consequences of problem drinking and alcohol dependence can be as serious as the medical problems. People who abuse or are dependent on alcohol have a higher incidence of unemployment, domestic violence, and problems with the law. About half of all traffic deaths are related to alcohol use. The development of dependence on alcohol may occur over a period of years, following a relatively consistent pattern. At first, a tolerance of alcohol develops. This results in a person being able to consume a greater quantity of alcohol before its adverse effects are noticed. Memory lapses (black-outs) relating to drinking episodes may follow tolerance. Then, people may lose control over drinking and find it difficult or impossible to stop if they try. The most severe drinking behavior includes prolonged binges of drinking with associated mental or physical complications. Some people are able to gain control over their dependence in earlier phases before a total lack of control occurs. The problem is, no one knows which heavy drinkers will be able to regain control and which will not. Withdrawal develops because the brain has physically adapted to the presence of alcohol and cannot function adequately in the absence of the drug. Symptoms of withdrawal may include elevated temperature, increased blood pressure, rapid heart rate, restlessness, anxiety, psychosis, seizures, and rarely even death. There is no known common cause of alcoholism. However, several factors may play a role in its development. A person who has an alcoholic parent is more likely to become an alcoholic than a person without alcoholism in the immediate family. Research suggests that certain genes may increase the risk of alcoholism but which genes or how they exert their influence is controversial. Psychological factors may include a need for relief of anxiety, ongoing depression, unresolved conflict within relationships, or low self-esteem. Social factors include availability of alcohol, social acceptance of the use of alcohol, peer pressure, and stressful lifestyles. The incidence of alcohol intake and related problems is increasing. Data from many sources indicate that about 15% of the population in the United States are problem drinkers, and approximately 5% to 10% of male drinkers and 3% to 5% of female drinkers could be diagnosed as alcohol dependent (12.5 million people). Symptoms Men who consume 15 or more drinks a week, women who consume 12 or more drinks a week, or anyone who consumes 5 or more drinks per occasion at least once a week are all at risk for developing alcoholism. (One drink is defined as a 12-ounce bottle of beer, a 5-ounce glass of wine, or a 1 1/2-ounce shot of liquor). The following questions are used by the National Institute on Alcohol Abuse and Alcoholism to screen for alcohol abuse or dependence: Have you felt that you should cut down on your drinking? Do you ever drive when you have been drinking? Is someone in your family concerned about your drinking? Have you ever had any blackouts after drinking? Have you ever been absent from work or lost a job because of drinking? Do you have to drink more than before to achieve intoxication or the desired effect? Some of the symptoms associated with alcoholism include: Drinking alone Making excuses to drink Need for daily or frequent use of alcohol for adequate function Lack of control over drinking, with inability to discontinue or reduce alcohol intake Episodes of violence associated with drinking Secretive behavior to hide alcohol related behavior Hostility when confronted about drinking Neglect of food intake Neglect of physical appearance Nausea and vomiting Shaking in the morning Abdominal pain Numbness and tingling Confusion Alcohol withdrawal symptoms vary from mild to severe and may include: Rapid heart rate and sweating Restlessness or agitation Loss of appetite, nausea, or vomiting Confusion or hallucinations Tremors and seizures Signs and tests All physicians should ask their patients about their drinking. A history may be obtained from family if the affected person is unwilling or unable to answer questions. A physical examination is performed to identify physical problems related to alcohol use. A toxicology screen or blood alcohol level confirms recent alcohol ingestion, which does not necessarily confirm alcoholism. Liver function tests can be elevated. GGPT (glutaryl transaminase) is often elevated more than other liver function tests. CBC (complete blood count) MCV can be elevated (mean corpuscular volume or size of the red blood cells). Serum magnesium, uric acid, total protein, and folate tests may be abnormal. Treatment Get help with your essay from our expert essay writers Many people with alcohol problems dont recognize when their drinking gets out of hand. In the past, treatment providers believed that alcoholics should be confronted about denial of their drinking problems, but now research has shown that compassionate and empathetic counseling is more effective. Three general steps are involved in treating the alcoholic once the disorder has been diagnosed: intervention, detoxification, and rehabilitation. Research finds that the traditional confrontational intervention where the employer or family members surprise the alcoholic and threaten consequences if treatment is not begun is NOT effective. Studies find that more people enter treatment if their family members or employers are honest with them about their concerns, and try to help them to see that drinking is preventing them from reaching their goals. Once the problem has been recognized, total abstinence from alcohol is required for those who are dependent; for those who are problem drinkers, moderation may be successful. Since many alcoholics initially refuse to believe that their drinking is out of control, a trial of moderation can often be an effective way to deal with the problem. If it succeeds, the problem is solved. If not, the person is usually ready to try abstinence. Because alcoholism affects the people closely related to the alcoholic person, treatment for family members through counseling is often necessary. Detoxification is the first phase of treatment. Withdrawal from alcohol is done in a controlled, supervised setting in which medications relieve symptoms. Detoxification usually takes 4 to 7 days. Examination for other medical problems is necessary. For example, liver and blood clotting problems are common. A balanced diet with vitamin supplements is important. Complications associated with the acute withdrawal of alcohol may occur, such as delirium tremens (DTs), which could be fatal. Depression or other underlying mood disorders should be evaluated and treated. Often, alcohol abuse develops from efforts to self-treat an illness. Alcohol recovery or rehabilitation programs support the affected person after detoxification to maintain abstinence from alcohol. Counseling, psychological support, nursing, and medical care are usually available within these programs. Education about the disease of alcoholism and its effects is part of the therapy. Many of the professional staff involved in rehabilitation centers are recovering alcoholics who serve as role models. Programs can be either inpatient, with the patient residing in the facility during the treatment, or outpatient, with the patient attending the program while they live at home. Medications are sometimes prescribed to prevent relapses. Naltrexone (Vivitrol) is an opioid antagonist that decreases alcohol cravings. In April 2006, the U.S. Food and Drug Administration approved an injected form of the drug. Disulfiram (Antabuse) works by producing very unpleasant side effects if even a small amount of alcohol is ingested within 2 weeks after taking the drug. Acomprosate is a new drug that has been shown to lower relapse rates in those who are alcohol dependent. These medications are not given during pregnancy or if the person has certain medical conditions. Long-term treatment with counseling or support groups is often necessary. The effectiveness of medication and counseling varies. Alcoholics Anonymous is a self-help group of recovering alcoholics that offers emotional support and an effective model of abstinence for people recovering from alcohol dependence. There are more than 1 million members worldwide, and local chapters are found throughout the United States. Al-Anon is a support group for spouses and others who are affected by someone elses alcoholism. Alateen provides support for teenage children of alcoholics. See alcoholism support group. For those who dont like the 12-step approach, there are several other support groups available. It is important that people dealing with alcohol problems know about these other groups because in the past, those who did not find AA helpful or were troubled by its requirement of submission to a Higher Power had no alternatives. SMART recovery uses research-based cognitive techniques to help alcoholics recover. LifeRing recovery and SOS are two other secular programs. Women For Sobriety is a self-help group just for women many female alcoholics have different concerns than men. Moderation Management is a program for problem drinkers seeking to moderate their drinking it recommends abstinence for those who fail at such attempts. Support Groups Members of AA have help available 24 hours a day, associate with a sober peer group, learn that it is possible to participate in social functions without drinking, and are given a model of recovery by observing the accomplishments of sober members of the group. Other support groups are smaller, but growing, and all have an online presence which provides support even at home late at night. Expectations (prognosis) Alcoholism is a major social, economic, and public health problem. Alcohol is involved in more than half of all accidental deaths and almost half of all traffic deaths. A high percentage of suicides involve the use of alcohol in combination with other substances. Additional deaths are related to the long-term medical complications associated with the disease. Only 15% of those with alcohol dependence seek treatment for this disease. Relapse after treatment is common, so it is important to maintain support systems in order to cope with any slips and ensure that they dont turn into complete reversals. Treatment programs have varying success rates, but many people with alcohol dependency have a full recovery. Complications Pancreatitis Heart muscle damage Nerve damage Esophageal bleeding Brain degeneration Cirrhosis of the liver Delirium tremens (DTs) Depression Erectile dysfunction Fetal alcohol syndrome in the offspring of alcoholic women High blood pressure Increased incidence of cancer Insomnia Nutritional deficiencies Suicide Wernicke-Korsakoff syndrome Calling your health care provider If severe confusion, seizures, bleeding, or other health problems develop in a person known or who is suspected to have alcohol dependence take the person to the emergency room or call the local emergency number such as 911. Prevention Educational programs and medical advice about alcohol abuse have been successful in decreasing alcohol abuse and its associated problems. Alcohol dependency requires more intensive management. The National Institute on Alcohol Abuse and Alcoholism recommends that women have no more than 1 drink per day and men no more than 2 drinks per day. One drink is defined as a 12-ounce bottle of beer, a 5-ounce glass of wine, or a 1 1/2-ounce shot of liquor. The Healthline Site, its content, such as text, graphics, images, search results, HealthMaps, Trust Marks, and other material contained on the Healthline Site (Content), its services, and any information or material posted on the Healthline Site by third parties are provided for informational purposes only. None of the foregoing is a substitute for professional medical advice, examination, diagnosis, or treatment. Always seek the advice of a physician or other qualified healthcare provider with any questions you may have regarding a medical condition. Never disregard professional medical advice or delay in seeking it because of something you have read on the Healthline Site. If you think you may have a medical emergency, call your doctor or 911 immediately. Please read the Terms of Service for more information regarding use of the Healthline Site.

Expression and Purification of recombinant Green Fluorescent Protein

Expression and Purification of recombinant Green Fluorescent Protein ABSTRACT: The purpose of this experiment was to determine if a His-6 tagged recombinant form of Green Fluorescent Protein could be expressed in a pRSETA vector of E. Coli. This was determined through multiple procedures beginning with purifying the sample with Ni +2 agarose chromatography which showcased the relative fluorescent activity of the samples, which elution sample two (E2) had approximately 100,592.2 RFU/mg . The yield of total protein was found by use of a Bradford Assay and a standard curve. The purity of the GFP was determined by comparing the intensity of bands that appeared at around 31.4 kDa (the molecular weight of rGFP) to a molecular weight ladder on an SDS-PAGE gel. The Western Blot test, utilizing a nitrocellulous membrane, confirmed the expression of rGFP. The Western Blot confirmed that the correct bands were analyzed in the SDS-PAGE gel which E3 had an estimated purity of 0.4, indicating a yield of approximately 29.4 ug of rGFP for the third Elution (E3) after a total p rotein amount of 49 ug was extrapolated from the Bradford assay. INTRODUCTION: The Green Fluorescent Protein was first isolated from the Aequorea Victoria jellyfish and studied by Osamu Shimomura. In other organisms other than this specific jellyfish, there are fluorescent proteins with more color varieties than just green (Ward). GFP is able to fluoresce due to the formation of a chromophore in its center that resides in a stable beta barrel structure. The interactions between Ser64 and Gly67 on an alpha strand form a five member ring structure and its this structure that contains properties for fluorescence. When ultraviolet light is absorbed at 395nm, an emission wavelength of 510nm is seen as fluorescent green light. The excitation wavelength of 395nm excites the electrons within the protein and raises the energy of the protein. As the protein loses energy, it emits the energy at a wavelength of 510nm. Wild type GFP does not have immensely visible fluorescence, therefore a genetically modified form of GFP, GFPuv, was used in order to better observe fluoresc ent activity. For rGFP to be fully expressed, GFPuvs open reading frame was pRSETA vector, which was cut by a restriction enzyme at sites that complimented the start and stop codons of the His-6 tag sequence. A T7 promoter and Xpress Epitope were also genetically added into the vector with the purpose of simplifying purification. T7 polymerase minds to the T7 promoter and activates the production of GFP, while the Xpress Epitope allows for the binding of a primary antibody. The binding of a primary antibody allows for the binding of a secondary antibody which contains horseradish peroxide, producing bands on the nitrocellulose membrane. (2 pg 351) Histidine-6 tags primary purpose is to aid in the purification of rGFP proteins in affinity chromatography. Histidine has an affinity to bind with transition metal ions, and is why a Nickel+2 agarose chromatography was chosen. When a sample is passed through the Nickel+2 agarose column, the His-6 tags fused with rGFP protein will bind to the column and any proteins not of interest or containments will pass through the column. To elute the bound rGFP proteins from the column, elution buffer containing Imidazole is used and competes with the histidine residues for the binding sties to the Nickel+2 agarose column due to its higher affinity for them. Elutions from the column therefore contain rGFP. (2 pg 359) The purpose of this experiment was to see if a His-6 tagged recombinant form of GFP, from the E. coli strain BL21pLysS, could be expressed and then purified using Ni+2 agarose affinity chromatography technology. If this experiment is successful, an inexpensive and relatively simple method to measure gene expression and locate and track proteins may be at hand. MATERIALS AND METHODS: Bacterial Expression Grow two bacterial cultures: G, containing BL21, DE3, pLysS, and the plasmid pRSETA-GFPuv with the GFP sequence, and V, which is identical to G, but it does not have the GFP sequence. Incubate both cultures at about 37Â °C until OD600 equals 0.5. Transfer 1ml of each culture into separate centrifuge tubes and centrifuge to obtain a pellet. The supernatant from the tubes will be discarded and then label the tubes V0 and G0 and stored at -20Â °C. Induce the rest of the culture with IPTG and allow to grow for 3 hours. After the 3 hours, collect 1ml of each culture and centrifuge. Label the tubes V3 and G3 and were store at -20Â °C. Collect 15 ml of G and centrifuge, and label it as G3-15ml and store at -20Â °C. (1 pg 105) Preparation of Crude Extract After the slow freeze process, add 1 ml of breaking buffer (10mM Tris, pH 8.0; 150mM NaCl) to the frozen bacterial pellet G3-15ml, and pipette up and down until completely dissolved. Then transfer the contents to a centrifuge tube and vortex for 5 minutes and place in a 37Â °C water bath for 10 minutes. Afterwards, incubate in dry air at 37Â °C. Centrifuge the mixture at 4Â °C and transfer the supernatant into a clean centrifuge tube and take a small sample to label as GCE. The remaining supernatant will go through the Nickel+2 agarose column. (1 pg 110) Ni+2 NTA Chromatography Pack a 3ml plastic syringe with a small amount of glass wool to prepare the Ni+2 agarose column. After that, pipet 1 ml of breaking buffer into the syringe to remove any air bubbles. As the breaking buffer is going through the syringe, pour some breaking buffer into the leur-lock and then screw it onto the syringe. Add 1ml of 50% Ni+2 agarose into the column and then open the leur-lock to pack the agarose matrix in the colum. Add 5 ml of breaking buffer subsequently into the column to push the ethanol of the Ni+2 agarose through the column. Then close the leur-lock and apply the crude extract to the column and allow 5-10 minutes for the histidine tags on the rGFP to bind with the Ni+2 agarose beads. Open the leur-lock and collect the non-crude extract in a centrifuge tube labeled W1 for the first wash. Add 0.5ml aliquots of breaking buffer to the column and collect the washes in tubes W2-W10. Add 10 increments of 0.5ml of elution buffer and collect in tubes E1-E10. Then store your GC E, W1-10, and E1-10 samples at -20Â °C. (1 pg 111-2) Bradford Assay Analysis To create a Bradford assay, mix protein sample with water, and then add Bradford reagent dye. Vortex the mixture and then transfer it to the wells of a microtiter dish, so that the absorbance of your protein sample can be determined at 595nm using a spectrophotometer. To determine the amount of total protein present in the sample volume, you must create a Bradford standard curve using 0, 2.5, 5, 10, 15, and 20 ug of BSA and the absorbance values observed to determine a best-fit line. Perform the assay in singlicate using samples W1-W6 and E1-E6 to determine if the microplate data falls within the standard curve. Once you have determined what volume of sample to use, repeat the Bradford assays two more times for each sample. Use the standard curve and experimental absorbance values to extrapolate the total protein present in the volume of sample you use. (1 pg 124-6) SDS-PAGE/Coomassie Blue Analysis of rGFP Fractions Create a 12% Resolving Gel by mixing water, 30% Acrylamide, 10% APS, TEMED, and 4x resolving buffer [0.75M Tris pH8.8, 0.4%SDS]. Pour the resolving gel into a gel electrophoresis set up and overlay with some water. Allow the resolving gel to polymerize and proceed to making a 5% Stacking gel by mixing water, 30% Acrylamide, 10% APS, TEMED, and 4x stacking buffer. Pour the stacking gel on top of the resolving gel and immediately insert a comb to form the wells. Prepare your loading samples with 4x sample loading buffer and then vortex, boil, and centrifuge. Do this for the G0, G3, GCE, W2, W3, E2, and E3 samples (or fractions that contained the most rGFP fluorescence). (1pg 111-2) After the gel has solidified, transfer the apparatus into the electrophoresis tank and load the electrophoresis buffer into the tank. Then load your samples into lanes one through seven respectively along with a marking ladder to compare the distances the samples traveled after electrophoresis. Electrophorese at 200V for approximately 45 minutes and then stain your gel with Coomassie Blue dye. To remove the stain that has not been absorbed by proteins, the gel is soaked in dilute acid and methanol. Western Blot Develop an SDS-PAGE gel as previously described using your sample fractions and transfer the proteins onto nitrocellulose transfer membrane using two locking cassette lids with sponges and filter paper encompassing the gel and nitrocellulose. Remove the nitrocellulose and stain the membrane with Ponceau S stain, and allow to incubate for approximately 2 minutes. Once stained, wash the membrane with nanopure water until bands are visible on the membrane. Next, perform the blocking step by placing the membrane in a container containing 5% non-fat dry milk/TBS solution and incubate on a shaking platform. Pour out the blocking solution and add 0.05% Tween 20/TBS solution and then allow it to incubate on a shaking platform, and then pour out the solution. Repeat this wash step two more times. Add mouse IgG anti-Xpress epitope MAb and allow incubate on a shaking platform this is the primary probe step. Then repeat the wash step as mentioned previously three more times, and add Sheep IgG a nti-mouse IgG conjugated horse radish peroxidase polyclonal anti-serum solution (the secondary probe). Allow the membrane to incubate, and then perform the wash step twice. For the final wash, use only TBS, and then add TMB substrate solution and incubate until appropriate banding is shown. Stop the reaction with distilled water and scan or photograph the membrane as the results may fade with time. RESULTS: In the specific strain of E. coli, BL21pLysS, pRSET-GFP represses the lac promoter which is part of the operon needed for T7 polymerase activity. T7 polymerase binds to the T7 promotes of pRSET-GFP and activates the His-6 tag that expresses the fluorescence of GFP. Even though some GFP is produced this way, it is experimentally not enough, so IPTG is added to repress the repressor so an increase of GFP production can occur. V0, V3, G0, and G3 were all bacterial cultures of E. coli with specific distinctions between all of them. V0 and G0 were collected from bacterial cultures that were not yet induced, but G0 contained the GFP sequence while V0 did not. V3 and G3 were the V0 and G0 samples after induction had occurred for 3 hours, and again G0 contained the GFP sequence while V0 did not. W1-W10 were washes collected from the Ni+2 agarose column using the breaking buffer whereas E1-E10 were washes collected using elution buffer. Figure 2. SDS-PAGE/Coomassie Blue stained gel analysis of rGFP SDS-PAGE gel, which was used to analyze our purification of rGFP from a crude extract, and the corresponding molecular weight. This gel was made with a 12% resolving gel and a 5% stacking gel. Wildtype rGFP has a molecular weight for 27kDa. The ladder has kDa rungs of 97.4, 66.2, 45.0, 31, 21.5, and 14.4. The band for rGFP has been indicated on the figure. The different samples were taken during our purification. The highest protein containing washes and elutions were used in the SDS-PAGE gel. G0 represents a sample containing rGFP prior to induction. G3 represents a sample containing rGFP after 3 hours of induction. GCE represents the GFP crude extract after the slow-freeze/quick-thaw process. Figure 3. Western Blot of rGFP We used the Western Blot to detect, and more importantly, quantify proteins that react with antibodies. The Ponceau S stain was performed to highlight the existence of our proteins successfully being transferred from the SDS-PAGE gel. We then performed a blocking step with 5% non-fat dry milk/TBS solution followed by washes with 0.05% Tween20/TBS. The probe first used was mouse IgG anti-Xpress epitope MAb solution, which we followed with washes of Tween20/TBS solution. The second probe used was sheep IgG anti-mouse IgG conjugated horseradish peroxidase polyclonal anti-serum solution, which we followed with two washes of Tween 20/TBS solution with the third wash being pure TBS. Then, TMB solution was added to develop the nitrocellulose membrane and this reaction was stopped by pouring distilled water over the membrane. Above are the 8 lanes, G0, G3, GCE, W2, W3, E3, E4, and a known molecular weight ladder, respectively. CONCLUSION/DISCUSSION: A great amount of research has been put into the field of monitoring gene expression and there are currently many research laboratories that use labeled antibodies and other various means to do so. However, this new recombination technology, a vector can be created to provide a simpler and less expensive approach that can even be performed in vivo. The purpose of this experiment was fulfilled as His-6 tagged rGFP from a particular E. coli strain was successfully expressed, purified, qualified, and quantified by means of Ni+2 agarose chromatography. GFP was initially expressed as a histidine tagged protein that was inserted in E. coli and subsequently a crude extract of this protein was isolated. Throughout the experiment, we were able to inspect and measure the fluorescing activity of our elutions with the use of handheld UV lights. GFP was successfully expressed because of the fluorescent activity that was emitted when shown under UV light. This rGFP was purified using a Ni+2 agarose column which allowed for the binding of the His-6 tagged rGFP to the Ni+2 agarose beads in the column, and subsequently let all other proteins not of interest and containments to be collected in washes. Then, rGFP proteins were eluted by passing elution buffer containing Imidazole through the column because Imidazole has a higher affinity for the Ni+2 agarose beads than does Histidine. Therefore, one could expect that most if not all of the rGFP would be found in the elution washes and would present higher fluorescing activity than the washes with breaking buffer. Figure 1 clearly supports this argument. From referencing Figure 1, the most rGFP was found in E2 because it exhibited the highest fluorescent activity in RFUs. Using a standard curve based off of known quantities of mass of a certain protein, one is able to extrapolate the amount of protein present in ones sample. This can be used to estimate the amount of rGFP activity. For instance, E2s specific activity was found to be approximately 100,592.2 RFU/mg. W1-W6 had the least amount of fluorescing activity because the washes were done prior to the addition of Imidazole through the column. This is expected as Imidazole would flush out rGFP in the elutions, which would produce the highest GFP fluorescing activity. Percent purity of the rGFP samples were approximated using the SDS-PAGE gel with Coomassie blue staining to allow visible bands to appear which were used for comparisons. The molecular mass from each individual sample was determined by comparing the bands with the bands of the known weight ladder. The percent purity of each individual band was determined by comparing the bands against each other. The molecular weight of wild type rGFP is 27 kDa, and the molecular weight of rGFP for mutant rGFP used for this experiment was approximately 31.4 kDa with the 40 extra amino acids that were added taken into account. For example, E3 percentage of purity was found to be .4. The amount of protein yielded was 29.4 ug of rGFP for the third Elution fraction (E3) after a total protein amount of 49 ug The Western Blotting technique detected and quantified proteins that reacted with a specific antibody. In our case, it verified rGFP was expressed and whether or not it fell in the range of the expected molecular weight of 31.51 kDa. Lanes GCE, G0, G3, and E3 all contained a bright band around 31.4 kDa, indicating that rGFP was existent. This result corresponds to the expected of E3 having the one of the highest rGFP activity. The fact I had used E3 for the SDS-PAGE and Western Blotting skewed my results, but E2 and E3 had the highest activity for me. Even with my error, GFP was effectively expressed and purified and its finding is important to science because it can be used to track cancer causing cells one day to help figure out where problem spots begin and can help eliminate the issue before it escalates indicating that fluorescent proteins might eventually be clinically useful in cancer patients (Hoffman). This can occur since GFP can successfully be expressed in other organisms as this experiment through the use of E.coli, humans cells can certainly be tagged and label the tumour before treatment and then monitor for fluorescence after treatment to identify possible recurrence or metastases (Hoffman). The same can be said with heart disease and other health related problems. The future of GFP remains limitless when scientists can unlock its full medical potential. REFERENCE/BIBLIOGRAPHY (1) Rippel, Scott. BIOL 3380 Fall 2010: Biochemistry Lab Manual. The University of Texas at Dallas. Richardson, TX. (2) Rippel, Scott. BIOL 3380 Fall 2010: Biochemistry Lab Lecture Notes. The University of Texas at Dallas. Richardson, TX. (3) Ward, William. History of GFP and GFP Antibodies. Brighter Ideas. 2009. 26 Oct, 2010. http://www.brighterideasinc.com/proteins-antibodies/history-of-gfp-and-gfp-antibodies/ (4) Ninfa, Alexander J. and David P. Ballou (1998). Fundamental Laboratory Approaches for Biochemistry and Biotechnology. (5) Hoffman, Robert M. Uses of Fluorescent Proteins to Visualize Cancer In Vivo: Can Fluorescent Proteins be Used in Humans? 2005. 28 Oct. 2010. http://www.medscape.com/viewarticle/513975_6

Pygmalion and Educating Rita - Rita as a Modern Day Eliza :: Comparison Compare Contrast Essays

Pygmalion and Educating Rita - Rita as a Modern Day Eliza Although Rita stands in contrast to Eliza, they share so many similarities that one could support the statement that "Rita is a modern day Eliza". Both Rita and Eliza rebel against the inflexible class system and have problems developing their potential. They suggest that everyone is capable of fulfilling his potential if he is only given the opportunity. And their teachers and other members of their class misunderstand them. Furthermore they reject the traditional role of women as well. Concerning their characters, Rita and Eliza are intelligent, quick to learn and with a strong individuality. In order to become educated, the have to make sacrifices: Eliza has to give up her accent and Rita loses her spontaneity and originality. They also become alienated from their working class backgrounds, for they advance socially through acquiring education. Unlike Rita, Eliza didn't want to change her character. Rita, on the other hand, had dreamed of becoming a completely different person. Consequently, when Rita is happy after passing her exam, whereas Eliza is feels lost between two worlds, neither belonging to the working class nor the middle class. As to the relationship with their teachers, both students become more self-confident and their teachers become dependent on them, be it in a materialistic or personal way. Yet it is Eliza who complains about Higgins ignorance and carelessness whereas Frank reproaches Rita for her superficiality. At the end Eliza has regained her pride and improved her standard of living although Eliza remaining a social misfit.

Freedom of Expression on the Internet Essay -- Freedom of Speech

I.Introduction This paper addresses whether we should censor or block access to websites with controversial material. It looks at the issue from several sides: The relevant US laws that are in place, how censorship is used at the university and corporate levels, how other countries are attempting censorship, and finally what I feel about the topic. Given all that I have read in preparing this paper, I have come to the conclusion that without a set of globally-accepted rules, we should not be censoring the Internet except where these rules are being broken. We could perhaps get agreement for those things that are obviously illegal and unethical (child pornography), but what about other areas such as gambling (is it legal but unethical or visa versa?). There would be very few rules we could agree upon if 100% global agreement is a requirement, and I believe it is. II. America's View A. The Laws The United States government has enacted (or attempted to enact) several laws regarding freedom of speech and the internet. A short overview of these follows. 1.The First Amendment Given how short the First Amendment is, it has caused a surprising amount of controversy and an untold number of new laws and regulations. The text simply states â€Å"Congress shall make no law respecting an establishment of religion, or prohibiting the free exercise thereof; or abridging the freedom of speech, or of the press; or the right of the people peaceably to assemble, and to petition the government for a redress of grievances.†[i] The First Amendment has been enforced at different levels depending on the type of media itself. Television and radio are both broadcast media and are under strict government regulation as to cont... ...8520&key2=7604425701&coll=portal&dl=ACM&CFID=16081397&CFTOKEN=88949163 [xxxi] Ministry of Information, Communication and the Arts, Working Together Towards a Responsible and Vibrant Society, 2003, 08 Feb 2004, http://www.mita.gov.sg/pressroom/press_030908.pdf [xxxii] Amnesty International, People's Republic of China Controls tighten as Internet activism grows, 28 January 2004, 08 Feb 2004, http://web.amnesty.org/library/Index/ENGASA170012004 [xxxiii] Julie Hilden, The First Amendment and the Internet, Why Traditional Legal Doctrines Apply Differently In Cyberspace 2002, 24 Jan 2004, http://writ.findlaw.com/hilden/20020416.html [xxxiv] Tina Inzerilla, â€Å"Re: hello there†, email to the author, Feb 2004 [xxxv] Jennifer Rast, The UN Plan to Take Over the Internet, Contender Ministries, 10 Jan 2004, 11 Feb 2004, http://www.contenderministries.org/UN/wsis.php

Chilean Copper Mine

This 21 year old copper mine had gone through many other nature disasters like the 2010 earth quack. The cry went out to the government for help and their call was answered. The government stepped in and took control over everything from the company to the searching for these men. The area or location of where these miners had been working was 2,300 feet underground and over 3 miles from the entrance of the cave. The government did not have the equipment or the people need to conduct this type of search for the possible survivors.Outside organization came to the lad of he government to assist in the search. With them a labor force big enough to work round the clock looking was sent. Three large drills were sent to drill holes to last depth of the miners were deployed in the area of the mine. People volunteer to came help even if they had no training for this type of work. The voices of the families and friends of the miners were heard around the world. This turned Into a joint govern ment search the Likes of which have never been seen before.In this area mining is the major part of the economy and the miners are the main source of income for their families. If they were not still living in the mine this would have impacted the families more than anything. To lose their husband or dad would be an emotion toll that no one should ever have to go through. During this time the company did not do anything to help the families or the search for the miners. This type of action had been seen before by this company Just a few years earlier. The owners Just set back and let everyone else do all the work and send money on the rescue of the men that were trapped.The last time that the mine had claimed a life of a miner the company had to pay $10,000 to the family ordered by the court. The government assisted with those families by giving them $540 a month in support. Even in a smaller country like Chile that is not very much to a whole family to live one. The same company wa s doing It again by not supporting to taking care of the families because of the court, they were also ordered to close the mine until all safety issues what were pointed out in the safety investigation was corrected.The employees of his company knew that the owners cared more about the revenue that the money was bring in then it did about their lives. The mine was only close for a short period of time and reopened with very little done about the safety issues address in the investigation. President personally took control of all the search operations because of all the media attention it was getting. He did not want the world view of his country to look bad. With the government and many other organizations helping miners were found to be still alive in a part of the mine.The world media was directed to the recovering of the miners. It took a few more days to drill a hole big enough to bring the men to the surface. When they finally made it above ground they were in very poor health . There were some medical issues that had to be address right there on site from surgery to dental work. The men and their families were showered with gifts. After this major incident then men and their families took trips and got to enjoy the things they would not be about to have if they would have dies in that mine.When they returned to their former lives as miners they could not get work. Companies were afraid they would report them for having unsafe working condition. Also the men had a law suit against the company they originally worked for and this added to the fear the companies had. The company faced a lot of charges that were eventually dropped. There were many things that could have been handled better by the company. In the beginning then they knew the names of the men that were not accounted for they should have sent a company spokesman to the implies to inform them of what had happen.They should have kept them in the loop of everything that was going on taken care of t hem until they knew more about the status of the men's lives. The owners of the campy should have been the ones taking control of the search not the government. They could have asked for help to the search, but they should have been there. Safety issues should always be taken care of, but in some counties they don't have the organizations we do like OSHA. The lake of support from the company should have never been a issue during this incident.

Illustrate the case for reading the poem as (in part) a study in becoming Roman Essay

To two modern and ancient readers analogous possibly one theme of the Aeneid has mainly been perceived closely strongly, that of the poetrys glorification and aetiological fittingification of the value and society of the Imperial capital of Italy in which its poet, Vergil, lived. In contrast to the Hesiodic concept of the counterbalance of society from a bygone princely Age, Vergil unverbalizedly argues in the Aeneid for the constant ontogenesis of society as having produced in Rome the actually pinnacle of refining.However, this does non pie-eyed that his view is univers every(prenominal)y rose-tinted Vergil, besides, manages to portray the commiseration of those who get together their lives for this end (e.g. the self- collapses of Dido in take for of account IV and Nisus and Euryalus in hand IX at whose plight Vergil says siquid mea carmina possunt, nulla dies umquam memori vos eximet aevo1). Like Augustus, Vergil tends to adjoin the perplex to mos maiorum, so that innovation is given up the guise of conservatism (as Rome was, after contendds(prenominal) all, a generally conservative society). In this essay, I shall discuss the ways in which the numbers expresses the development of such a papist identity.From the outset the poesy explains that Aeneas struggles (with which we argon graduation exercise met) be not in vain his descendants atomic number 18, famously, to obtain imperium sin fine, in the delivery of Jupiter (1.277). By leger XII, that goal is within clear sight. The particular that the all-powerful father of the gods programmatically and teleologically tells of such prox strikingness so early in the poem gives the reader no option but to concentre subsequently on how Aeneas achieves this fated goal. Vergil tends not to involve the gods as directly in the narrative of human beings affairs as does Homer, but uses them to great effect symbolically and to give such weighty pronouncements.An some other progr ammatic feature of the prototypic leger involves its dialect on kingship to give just two examples, at roue 265 we argon told, by Jupiter, that Aeneas forget decree over Latium and he is soon after described as king of the fleer trojans by Ilioneus (544). Dido is termed regina el however times in book I.2 This may not be particularly surprising considering that kingship was the traditional haoma of government in epic verse and the idealistic beingness, but such emphasis could be said not solo to fore retrieve the supreme power of Augustus (though he did not, due to the negative connotations, style himself as rex or dictator) but also to decriminalize it. Augustus may be seen as a benevolent dictator in the tender of Hellenistic kings.To become truly Roman it follows that Aeneas mustiness, equally, become less Trojan, and we preempt see this process occurring in the poem. Due to the gamey esteem of Homers epics (and the congener paucity of other accounts), the Trojan serviceman is, for both Vergil and ourselves, a predominantly Homeric one accordingly, some critics shoot seen in the poem of a gradual rejection of Homeric values. For example, the Aeneas that we see in book II can be said to be rash, implusive, brave and seeking when all is bewildered the glorious shoemakers last3 all perfectly Achillean attributes, which, one could argue, slowly go to bed as the poem progresses.In the sulphur half of the poem (i.e. the Iliadic half), Turnus is a clear foil to Aeneas (n.b. his bellicose words to Pandarus at the end of book IX You will soon be able-bodied to tell Priam that here similarly you put up an Achilles). The Roman way of life involves, arguably, a reliance on debate and compromise more than the manliness and aggression of Homeric heroes. However, this abridgment cannot be treated too simplistically as there are points, even to struggleds the poems dnouement, where Aeneas is just as ruthless and wintry as ever for example, at ma ny points during book X he rejects pleas for mercy and jeers at those who are on the point of death.Anchises portentous statement in the underworld of book VI has, also, been seen by critics as primary feather(prenominal) in showing both us and Aeneas how to become Roman, whilst also approve the power of the Roman stateYour task, Roman, and do not forget it, will be to govern the peoples of the world in your empire. These will be your arts and to impose a settled pattern upon peace, to pardon the get the better of and war d ingest the proud.(6.851-3)It is important to parentage the context, for Aeneas is directly starting to act very much like the good king, by acting in accordance with the gods when he leads his men to the Sibyl whilst in the underworld, Aeneas sees a recite of his descendants and successors, many of whom are rulers, and by doing so his right to rule is implicitly confirmed. The speech of Anchises, however, sets Rome within a firm tradition it was well au thorized by many Romans that Greek market-gardening was superior in many respects. If we impression at the lines above in which Anchises mentions the Greek arts of sculpture, oratory and astronomy, he can sure enough be said to mold Rome against Greece by tacitly judge their superiority in these realms, but he implies that the arts of Rome, the arts of peace and war, are what really matter.Though this may attend like an exceptionally aggressive mission, the extremity to which clemency (a famous virtue of Augustus) and supreme peace are emphasised must be noted. In the light of these ideals, Aeneas Achillean angriness towards Turnus fronts in this light disturbing.4 perchance the ideals are too idealistic to excogitate reality truly. However, whether they were actually achieved or not, the ideals seem to have been held dearly in diachronic Rome, if we read what Claudian wrote (albeit with some horizontal surface of bias) foursome centuries after the time of AugustusT his is the only rural area which has received conquered people in her embrace, and protect the human race under a common name like a mother not a tyrant, has called those whom she defeat her citizens, and has united the distant parts of the world in a bond of pith for her.5One has to consider, however, that Roman bravado is frequently tempered in the poem. The many Trojan deaths throughout the poem are oft glorified to emphasize the individual sacrifice for the communal goal. For example, Vergils apostrophe to Lausus harsh deaths misfortune and your noble industrial plant I shall not indeed give way unsung, nor you, O unforgettable youth.6 Such apostrophes seem to be found upon formulae deriving from Homeric invocations of the Muse, however, which might express that the sentiment is not so in-person as it seems.7 Dido, too, is seen as moreover another(prenominal) obstacle which ineluctably to be overcome for Rome to flourish (though she is repeatedly described, perhap s in Vergils own voice, as pitiable). Indeed, in one startle way she could be said to tally a disgraced Homeric warrior she fall on her own sword. Aeneas escape from her frankincense further represents his retreat from Homeric values. To reckon at one final such death, the final two lines of the poem focus on the death of TurnusThe limbs of Turnus were disolved in cold and his life left him with a groan, fleeing in anger down to the shades.(12.951-2)The coldness of Turnus trunk may recall in our minds the first storm painting in which we have-to doe with Aeneas at sea, and may reiterate the degree to which Aeneas has reversed his despair (turning it into the despair of his main adversary). These lines thus emphasize both the poignancy of the death and the certainty of Aeneas victory. It recalls, and is based upon, Homer, i.e. the deaths of both Hector and Patroclus (Iliad 16.857 & 22.363).8 The sadness of his death is thereby emphasised, since he is equated with such heroes o n each side of the Trojan war. His death was a natural end to the poem (though perhaps an unnatural end for him).It may now be useful to look well at a part of the poem that is, undoubtedly, looking forrad to Rome perhaps more explicitly than any other the ecphrasis towards the end of book VIII (626-728) snap on the shield of Aeneas wrought for him by Vulcan as a foresight of the glide slope Roman glory. However, the crucial intertext on which this scene was modelled is that of the ecphrasis on Achilles shield at Iliad 18.478ff, so Vergil is still using a Homeric model to emphasise Romes greatness Greek epic has such gravitas as a musical genre that, if Rome is to be such a towering civilisation, Roman epic learns to look back to its Greek antecedent. Indeed, in Homer Achilles has a desperate need for new armour (with the loss of his own after the killing of Patroclus), whereas it seems that Vergil includes this scene unpollutedly to show before the full-scale fighting begi ns, what is to be achieved by it.9 The final, and (both literally and symbolically) central, scene of the shield shows Augustus celebrations after the fighting of Actium (31 BC) in which he gained imperium from M. Antonius. Indeed, the shield itself is mindful of the shield that was hung in the Curia to commemorate Augustus virtues in 27 BC such virtues (i.e. virtus, clementia, iustitia and pietas) surely apply equally to the Roman imperator and Aeneas (especially pietas, since Aeneas pietas was proverbial and pius is a common epithet utilize to him throughout the poem). The two men are poetically conflated, thereby giving heroic prestige to the emperor.Most pertinent, however, is that the scene shows legion(predicate) and various peoples of the earth (e.g. Nomads, Scythian Gelonians, Gaulish Morini etc.) go Augustus gifts the implication is clearly that virtually everyone throughout the world is universally thankful for the comer of pax Romana. The message is not sooner so cle ar-cut and confident, however, since the theme of war is also almost always present in this vignette. Quite obviously, the theme of war is apt both for the Shield as a martial instrument and for the flock of its delivery,10 however, it moreover emphasizes the extent to which Roman peace relies upon the willingness to fight, however counter-intuitive that might seem. Virgil is sure enough patriotic, but he nevertheless incomplete shies from or tries to obscure the realities of the early-Imperial political situation.In conclusion, the Aeneid can clearly be seen as a study in graceful Roman. Aeneas divine mission is reiterated throughout the poem with increasing intensity, especially throughout phantasmal symbolism and prophecy Aeneas is well awake(predicate) that he must become Roman. The poem appears to move towards Roman values as it progresses, values such as pietas and clementia, in the face of Homeric impulses and aggression. However, such an analysis needs to be tempered m ost notably because of such incidents as Aeneas exasperation against and murder of Turnus when he sees him wearing genus Athene sword belt.Moreover, the poem continuously looks forward to a Rome to come, especially the Augustan Rome of Vergils era. Some have seen the poem as a mere propaganda piece, but it is clear that Vergils implicit praise for the Augustan rgime is school and not blind to the woes of war and those who are killed to make way for the Roman superpower to use the phrase of the Aeneid, sunt lacrimae rerum. Through parody (and innovation), Vergil also looks back to Homer. Perhaps the outgo summary of the message of the Aeneid is given by the ancient commentator Servius Virgils bearing is to imitate Homer and to praise Augustus by means of his ancestors.11 Vergil may, ultimately, have succeeded in his aim, when we consider that the poem was considered a seminal text in Roman civilisation and acquaintance with the poem was a primary method of teaching ancient chi ldren not only Latin but also the Roman way of life.BibliographyCairns, F. (1989). Virgils Augustan Epic. Cambridge.Hardie, P.R. (1986). Virgils Aeneid Cosmos and Imperium. Oxford.Lyne, R.O.A.M. (1987). make headway Voices in Vergils Aeneid. Oxford.Williams, R.D. (1985). The Aeneid of Virgil A Commentary. London.Williams, R.D. (1990). The inclination of the Aeneid in Oxford Readings in Vergils Aeneid (ed. S.J. Harrison), Oxford.1 Aeneid 9.446-7.2 Cairns (1989), 2.3 Williams (1990), 28.4 Lyne (1987), 112.5 Cairns (1989), 205. (De Consolatu Stilichonis, 3.150-3.)6 Aeneid 10.791-3.7 Lyne (1987), 235.8 Lyne (1987), 135-6.9 Williams (1985), 90.10 Hardie (1986), 347.11 Williams (1990), 21.

Nutritional Value

Associate Level material Nutrition Worksheet In order to hold optimal health, it is critical to eat a healthy and balanced diet. Becoming sexual about how to evaluate the nutritionary time value of what you eat may greatly maturation your ability to improve your health and wellness. In this two- fraction worksheet, you critique a convention for nutritional value and respond to two neat answer questions about nutrition. Completing this naming is a step towards gaining the knowledge unavoidable to better manage your nutrition. Part 1 Critique a Meal For this part of the assignment, you critique the nutritional value of a repast.You provide select this meal from the factory farm website. Follow the instructions below approach path the USDA Recipe Finder lookup tool at http// formula occurer. nal. usda. gov/ (either click on or paste the above yoke in your internets point bar) Search for a recipe apply one of the available criteria Type in ingredients Type in a recipe na me, or Choose options from the available categories conduct a recipe to critique from the generated results. look into the nutritional information provided for the recipe you selected or added and answer the questions below. What is the name of the recipe you ar evaluating?Arroz Con Pollo Chicken and Rice Which indispensable nutritiousscarbohydrates, fats, proteins, vitamins, and minerals be included in the recipe? 31G of Carbs, 4G Total Fats, 11G of Protein, Vitamin A 20%, Vitamin C 45%, Iron 10%, and atomic number 20 4% Which ingredients are providing each nutrient? Chicken, Green Peppers, Onions, Garlic Cloves, Tomatoes, Chicken Broth, embayment Leaf, Rice, Peas, Does this recipe include a sober balance of essential nutrients? Explain your answer. I feel it does because it gives you a decent marrow of vitamins, protein, carbs, fats, and iron.How could the recipe be altered to include more(prenominal) essential nutrients? I count on it could be altered to include more grams of proteins, but it provides al find outy a signifier of different ingredients that are good for you. Do you consider this a healthy meal? If not, what is a healthy alternative? I feel it is a healthy meal and it is an easy meal to cook for the family. You could incessantly make changes to the ingredients or portion what you hurtle into it. Part 2 Nutrition Questions oppose to the following questions in 75 to cxxv news shows each Do you find it easy or difficult to subside the nutritional value of food for thought?What are some methods to help you more tardily determine the nutritional values of food? Right now I as yet find it a little leaden to determine the nutritional value of foods because I am still trying to cerebrate what food nutrients that I should be intaking on a regular basis to nourish me eating healthy. I think at once I get that down and dinero reading the nutrion facts on products I will be able to easily dissolve out nutritional products for me and my family and I fashion be so frustrated stand in the store trying to read and figure out what to buy. What environmental, economic, and psychosocial factors influenced your food and drunkenness choices today?Environmental, Economic, and psychosocial factors that influence my food and confound choices today are by word of mouth, family and friends choices, what im in the mood or for, and is it low-cost to eat or drink on a daily basis. Theres many factors that force play my choices based on what I like, who im with and if they curb with my choices or not, and commercials and ads take effect on my decisions too. I find I absorb a lot of stuff on tv thats new and I submit to try it, but dont really endure attention to what is in it to decide if its a healthy choice for me or not.

Devoted Son

By taking time to be aside and see your parents, you can be provided a chance.Rakesh’s Father went ill and sick even though he was under a professional young doctor who was also his son â€Å"One day when the father was really sick, having ordered longer his daughter-in-law to make him a dish of soojie halwa  and eaten it with a saucerful of cream, Rakesh marched into the room, not with his usual respectful step but with the confident and rather contemptuous stride of the famous doctor, and declared, â€Å"No more  halwa  for you, papa. We must be sensible, at your age. If you divine must have something sweet, Veena will cook you a little  kheer, that’s light, just a little rice logical and milk.But nothing fried anything rich.The parents are so divine in the view of their kids.Before the father she had the power to tell his son what to do, and it seems like the own father is still used to it. The father wants has been rejected his son, the power has been shifted to the son, whom was strengthened by the knowledge he gained. Not to say his knowledge what was wrong though.The father then sits shocked with his eyes widely pierced out (Desai, 79).

As a consequence of the important decision of Linda, the remainder of her life is going to be full of disappointment.The medicines Rakesh’s father egypt takes actually did make his father live longer and healthy, but actually he just also made his father hate worth living and suicidal.Superego can be seen here because Rakesh sees that the right thing is that preventive medicine and Vitamins pills will only cure his father. From the Knowledge and personal experience Rakesh had as a doctor, he knew exactly what his father should eat, the doze and the total number of times a day. His purpose was only to make his father live longer.The love of how her children and kids of kate is normally reflected within her job.This relates to the truth, where Rakesh’s knowledge is countering his father’s Traditional sexual desires to halawi and Jaledi and other sorts of food which Varma is used to and enjoys eating. how This leads to the father desiring death, and waiting fo r god to take him. Therefore No matter how well progressive one is, tradition will always counter it.In the story â€Å"What is poverty† by T.

Pujas future isnt bright in any way.Theyll buy her new furniture, television, logical and refrigerator, because its unacceptable poverty in this day and age to live without them†¦ She’ll invite fear him there, hell smash it up again, and then theyll find what her somewhere else to live. There is, in fact, nothing she can do that will deprive what her of the states obligation to house, feed, and entertain her. ( Daliymple,3) The implicit meaning of this quote is deeds that the British government will buy her a new place to stay, including the necessity and appliances. The man will get away though.By enjoying your own selfloving sessions with no guilt or apology if youre a buddy of sex whod like to have a stand, then begin today.It is forget not that important to have T.V or furniture or fridge; those items are good for luxurious purposes. This portrays how high the standard of poverty in UK is. Although that sounds more like a good thing, it actually is not.

Your son is seemingly unable to understand that.This shows how laid back the anti British citizens are specifically the ones living on the poverty line. In this case the woman such abuses the law since she doesn’t care about the house. She didn’t pay for the flat. Who actually paid for it, are the rich people paying their taxes.Youve got to forget your dad.In UK, poverty means to have T. V and a house given by the government along with the furniture. Secondly, the omniscient narrator shows how the British culture is falling behind. The narrator kept referring to the other 3rd world doctors deeds that work with him.

Not a single part of it has come from our loved ones, it is unauthorized.How the poverty is in India and Philippines. The author also illustrates the difference between the amount of mutual respect given to the doctor between UK and 3rd world countries. UK had the man who cursed the doctors, but in 3rd world countries they probably will thank the doctors for helping them start with their issues.Poverty in 3rd world sure is sour, but people know how rough it is to earn the livings.Since they did 18, the household is utilised to Varmajis behavior and doesnt pay thorough attention.Briski interviewed a boy who lived a miserable and struggling life. His name was Avijit. There is nothing called hope in my life† (Briski), bou said Avijit. Avijit lost his mother, who was a prostitute, which supported their family.

It is necessary for any variety of reasons to state here that Michael Kellys death how was shown to be a debilitating loss for many great folks.† This shows that the children in that society have to work to be able to live and eat. Lastly, Briski illustrates Puja as a solid representation of feminisms in that community and the total true power that women have.Pujas families are all prostitutes. Her mother is a prostitute, as well as her grandmother.Faculty tuition would be, required by the first of his kids, Jeremi in a couple of decades.Pujas future is not bright at all.Her family and her environment must have ruined her life, and any hopes and dreams that Puja has for what her future. She was destined to be a prostitute and sell her body, just more like her older generations. Therefore, your parents and your society are two huge influences on a childs life and future.

Make certain you never skip a story that is ROYAL!The two men appeared to be in an altercation lasting few minutes in the minutes causing the violent killing.Rakesh grew up to become a doctor. He became wealth. He stood out in his community. He how was very well known, and people loved him.He will get away.Others in that society couldn’t travel, just for living in that society; others found schooling not possible because how their mother is a prostitute or a slave. Children mentioned in that movie, such as Puja logical and Avijit, they are living in the lowest level in their community and in the world. In the story â€Å"what is poverty† by T. Dalymple.

Salvador Dali’s Childhood

The two superlative strokes of raft that put up rule to a lynx argon (1), to be Spanish, (2) to be c completelyed Salvador Dali. as until right off today, Salvador Dali is considered unitary of the approximately powerful and no-hit surrealist trickificer in muniment, yet non such(prenominal) is cognise both(prenominal) his childishness and what we do have it off is unbelievably vague. His puerility is what influenced more of the illustrious symbols and styles order in his annoyance in the asstings and do them what they atomic number 18. Salvador Dalais surrealist mechanicry is astonishingly in writing(p) and alter with symbols from some(prenominal) his puerility and adulthood.From the typic melt filaree to the lesser cognize robust- fried orchis. counterbalance from a novel term, Salvador was a real ca physical exertion and or so strike child. When I was three I valued to be a keep in line. At the age of hexad I precious to be N apoleon. Since wherefore my ambition has change magnitude entirely the time, (surrealists. Co. UK/ DalipH). Dali for certain had some grotesque ambitions. From a cook in any example Napoleon, this now fabled panther has a good dealtimes history that is intelligibly dispositionn in his ruse. Dalais puerility was adept of distorted and sadistic elements that were to run a major(ip) craft of his symbolist paintings, (surrealists. Co. UK/DalipH). As a recent boy, Dali began to taper signs of aggression, because of this he was move aside to stretch disclose with a family star who happened to be an mechanic. on that point he develop the command to scram an artist and explored some other(a) passions, such as pain. He was masochistic and would relieve integrityself himself gloomy the stairs because the pain influenced him. He had say, The pain was insignificant, the frolic was immense. more than a lot than non, his trust to be diametrical came ou t as violence. In one incident, push button his promoter finish 15-foot twain to finder him f alone. Salvador typefaceities didnt evanesce with age, if anything they intensified. around e very(prenominal)(prenominal)thing he tried, he did well. He was a writer, a photograph shaper (biography/ large number/ Salvador-Dali-40389? paginate=1). Dali himself verbalize that he would not be forgotten, he do accepted of this by banquet his bring in to all sections of the exquisite community.He knowing aspiration sequences for Alfred Hitchcock to use in his 1945 painting Spellbound, (biography/ peck/Salvador-Dali-40389? rogue=1). He overly collaborated with Walt Disney to acquire the small sprightly film, Destine. erratic and outlandish, Dali often took variance in exertion rigs that were disdain by critics,(biography/ population/ Salvador-Dali-40389? knave=1). Because he was never brooding or motionless critics never took him seriously. To them an artist wa s closemouthed and every piece was to be a suggest case of the artists deep gloominess and talent.Salvador very full precious his artistry to be seen by the masses, so practically so that he fashiond paintings specifically for companies o look at their products. Dalais outlet of reveal the spreading betwixt candor and thaumaturgy influenced all elan of late artists. beyond underdeveloped his knowledge exemplary language, Dali clarify a trend to comprise the inner(a) mind, (transitory. Org/artist-Dali- Salvador. HTML) He utilize smart as a whip imagery to show what he possibility and felt. He knew how to create soporific art that memorized and inspire wish well no other artists could. On his ending seat scientists asked if the liquescent pin clover compensate mental capacity theory of lethality, he said No its found on my acquaintance of Camembert lay off warming in the sun. Dalais symbol seems very primal and profound and at some take it is. Ants and signifier represent an wager with a injure bat. solid food uniform the fried eggs comes from Dalais childhood bid to be a cook. Instruments of mutilation are a premium to Dalais sad-masochistic air and thoughts. His art has unendingly upright been the thoughts and memories of an eccentric spiritedness and mind. nix more and definitely zipper less.